医学教育改革系列教材 病原生物学与免疫学实验 英文版
作者：张力平
出版时间：2012年版
丛编项： 医学教育改革系列教材
内容简介
This laboratory text is used to guide the students'learning and training of microbiology，parasitology and immunology lab techniques，procedures and experiments.The outcome expected is students'capacity of doing research in laboratory of microbiology， parasitologyand immunology.The manual consists of two parts：PartⅠ——outlines the 8 basic experiments.PartⅡ——outlines the 21 comprehensive experiments of microbiology，parasitology and immunology.The involved experiments are designed to help enhance and deepen the students'un-derstanding of microbiology，parasitology and immunology.
目录
Part 1 Basic Experiments
Experiment 1 Observation of Bacterial Morphology
Ⅰ.Using Oil Immersion Lens of the Microscope
Ⅱ.Basic Morphology and Special Structurc of Bacteria
Experiment 2 Bacterial Staining and Microscopy
Ⅰ.Gram Stain
Ⅱ.Acid-fast Stain（Ziehl-Neelsen Method）
Experiment 3 Preparation of the Basic Medium
Experiment 4 Examination Methods of Parasitic Infections
Ⅰ.Direct Fecal Smear Method for Detecting Ascaris Eggs
Ⅱ.Examination for Ascaris I.arvae in Lung Tissue of Mice
Ⅲ.Saturated Brine Flotation Method for Detecting Hookworm Eggs
Ⅳ.Fecal Culture Method for Hookworm Larvac
Ⅴ.Cellophane Tape Method for Detecting Pinworm Eggs
Ⅵ.Direct Sputum Smear or Centrifugal Sedimentation Method for Detecting Paragonimus Eggs
Ⅶ.Squash Method for Detecting Striated Muscle Larvae of Trichmella
Ⅷ.Test of Concentration of Eggs by Sedimentation for Detecting S.Iaponicum Eggs
Ⅸ.The Miracidium Hatching Method for Detecting S.Japonicum
Ⅹ.Circumoval Precipitation Test（COPT） for Diagnosing Sc.histosomiasis
Ⅺ.Direct Stool Smear with Iodine-stain for Detecting Amoeba Cysts
Ⅻ.Blood Smear Method for Detecting Malarial Parasites
Experiment5 Agglutination Reaction
Ⅰ.Dircct Agglutination Reaction-Identification of the ABO Blood Group System
Ⅱ.Indirect Agglutination Reaction——-Anti-streptolysin O Test
Ⅲ.Indirect Agglutination Inhibition Reaction Latex Pregnancy Test
Experiment 6 Precipitation Reaction
Ⅰ.Single Radial Immunodiffusion Test
Ⅱ.Doublelmmunodiffusion Test
Experiment 7 Enzyme-Iinked Immunosorbent Assay（Sandwich ELISA Assay）
Part 2 Comprehensive Experiments
Unit 1 Isolation of Microorganisms from the Environment and Human Body
Experiment 8 Bacteria in the Air and Ultraviolet Radiation
Experiment 9 Bacteria on the Finger Tip and Solid Surface
Unit 2 Detection of Skin Infections
Experiment 10 Cultural Characteristics of Bacteria and Fungi（Teaching Demonstration）
Ⅰ.Clostridium Perfringens in the Milk Medium.
Ⅱ.Fungiin Sabouraud Dextrose Medium
Experiment 11 Observation of Plasmodium,Toxoplasma Gondii,Trichinella Spiralis,Echinococcus Granulosus and Insecta
Ⅰ.Plasmodium
Ⅱ.Toxoplasma Gondii
Ⅲ.Trichinella Spiralis
Ⅳ.Echinococcus Granulosus
Ⅴ.Insecta
Unit 3 Detection of Respiratory Infections
Experiment 12 Isolation & Identification of Pyogenic Cocci
Ⅰ.Isolation and Culture of Mimic Pus Swab and Throat Swab
Ⅱ.Coagulase Test
Experiment 13 Cultural Characteristics of Respiratory Infected Bacteria
Ⅰ.The Colonies of Pathogenic（Jocci on Blood Agar Plates（Teaching Demonstration）
Ⅱ.Mycobacterium Tuberculosis in Lowenstein Jensen Solid Medium（Teaching Demonstration）
Experiment 14 Observation of Paragonimus Westermani
Unit 4 Detection of Enteric Infections
Experiment 15 Isolation and Identification of Pathogenic Enterobacteria
Ⅰ.Isolation of Artificial Fecal Specimens
Ⅱ.Biochemical Activities of Enteric Pathogens-Kligler Iron Agar Test
Ⅲ.Serologic Identification of Enteric Pathogens
Experiment 16 Widal Test
Experiment 17 Observation of Ascaris lumbricoides,Hookworms,Enterobius Vermicularis,Taenia,Entamoeba and Cr yptos poridium
Ⅰ.Ascaris Lumbricoides
Ⅱ.Hookworms（Ancylostoma Duodenale and Necator Americanus）
Ⅲ.Enterobius Vermicularis
Ⅳ.Intestinal Cestodes（Taenia Solium and Taenia Saginata）
Ⅴ.Intestinal Protozoa（Entamoeba Histolytica and Entamoeba Coli）
Ⅵ.Cryptosporidium
Unit 5 Detection of Hepatic Infections
Experiment 18 Observation of Clonorchis Sinensis
Experiment 19 Observation of Schistosoma Ja ponicum
Unit 6 Detection of Genitourinary Tract Infections
Experiment 20 Observation of Chlamydia Trachomatis Inclusion
Unit 7 AntimicrobiaIResistance
Experiment 21 Bacterial Conjugation:the Transfer of Plasmids Coding for Antibiotic Resistance
……
Appendix
Index
